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key components  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc key components
    (A to C) The transverse and longitudinal migration ability of nestin + -BMSCs were measured by scratch assay and transwell assay, respectively. (D) The proliferative activity of nestin + -BMSCs was detected by CCK8. (E & F) Alcian blue staining, alizarin red staining and gene expression levels detected by qRT-PCR were used to analyze the chondrogenic and osteogenic differentiation potential of nestin + -BMSCs. (G) The expression of primary cilia in nestin + -BMSCs was detected by ace-tubulin fluorescence staining. Bar indicated 20 μm. (H) Expression of actin in nestin + -BMSCs stained with TRITC labeled Phalloidin Rhodamine. Bar indicated 50 μm. (I) The expression of acive-YAP in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (J) The expression of TAZ in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (K & L) Expression of key <t>components</t> of Hippo signaling pathway in nestin + -BMSCs. *, p < 0.05; #, p < 0.01.
    Key Components, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/key+components/pmc12302343-114-5-12?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    key components - by Bioz Stars, 2026-07
    86/100 stars

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    1) Product Images from "The effects of primary cilia-mediated mechanical stimulation on nestin + -BMSCs during bone-tendon healing"

    Article Title: The effects of primary cilia-mediated mechanical stimulation on nestin + -BMSCs during bone-tendon healing

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2024.09.012

    (A to C) The transverse and longitudinal migration ability of nestin + -BMSCs were measured by scratch assay and transwell assay, respectively. (D) The proliferative activity of nestin + -BMSCs was detected by CCK8. (E & F) Alcian blue staining, alizarin red staining and gene expression levels detected by qRT-PCR were used to analyze the chondrogenic and osteogenic differentiation potential of nestin + -BMSCs. (G) The expression of primary cilia in nestin + -BMSCs was detected by ace-tubulin fluorescence staining. Bar indicated 20 μm. (H) Expression of actin in nestin + -BMSCs stained with TRITC labeled Phalloidin Rhodamine. Bar indicated 50 μm. (I) The expression of acive-YAP in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (J) The expression of TAZ in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (K & L) Expression of key components of Hippo signaling pathway in nestin + -BMSCs. *, p < 0.05; #, p < 0.01.
    Figure Legend Snippet: (A to C) The transverse and longitudinal migration ability of nestin + -BMSCs were measured by scratch assay and transwell assay, respectively. (D) The proliferative activity of nestin + -BMSCs was detected by CCK8. (E & F) Alcian blue staining, alizarin red staining and gene expression levels detected by qRT-PCR were used to analyze the chondrogenic and osteogenic differentiation potential of nestin + -BMSCs. (G) The expression of primary cilia in nestin + -BMSCs was detected by ace-tubulin fluorescence staining. Bar indicated 20 μm. (H) Expression of actin in nestin + -BMSCs stained with TRITC labeled Phalloidin Rhodamine. Bar indicated 50 μm. (I) The expression of acive-YAP in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (J) The expression of TAZ in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (K & L) Expression of key components of Hippo signaling pathway in nestin + -BMSCs. *, p < 0.05; #, p < 0.01.

    Techniques Used: Migration, Wound Healing Assay, Transwell Assay, Activity Assay, Staining, Gene Expression, Quantitative RT-PCR, Expressing, Fluorescence, Labeling, Immunofluorescence

    (A & B) Inhibitory effect of actin on active-YAP and TAZ expression in nestin + -BMSCs. Bar indicated 50 μm. (C & D) Inhibitory effect of actin on expression of key components of Hippo signaling pathway in nestin + -BMSCs. (E) The effect of inhibiting Hippo signaling pathway on nestin + -BMSCs’ proliferation activity was detected by CCK8. (F to H) The effects of Hippo signaling inhibition on nestin + -BMSCs’ migration were detected by scratch assay and transwell assay. (I & J) The effects of Hippo signaling inhibition on nestin + -BMSCs’ differentiation potential were detected by alcian blue staining, alizarin red staining and gene expression levels. *, p < 0.05; #, p < 0.01.
    Figure Legend Snippet: (A & B) Inhibitory effect of actin on active-YAP and TAZ expression in nestin + -BMSCs. Bar indicated 50 μm. (C & D) Inhibitory effect of actin on expression of key components of Hippo signaling pathway in nestin + -BMSCs. (E) The effect of inhibiting Hippo signaling pathway on nestin + -BMSCs’ proliferation activity was detected by CCK8. (F to H) The effects of Hippo signaling inhibition on nestin + -BMSCs’ migration were detected by scratch assay and transwell assay. (I & J) The effects of Hippo signaling inhibition on nestin + -BMSCs’ differentiation potential were detected by alcian blue staining, alizarin red staining and gene expression levels. *, p < 0.05; #, p < 0.01.

    Techniques Used: Expressing, Activity Assay, Inhibition, Migration, Wound Healing Assay, Transwell Assay, Staining, Gene Expression



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    (A to C) The transverse and longitudinal migration ability of nestin + -BMSCs were measured by scratch assay and transwell assay, respectively. (D) The proliferative activity of nestin + -BMSCs was detected by CCK8. (E & F) Alcian blue staining, alizarin red staining and gene expression levels detected by qRT-PCR were used to analyze the chondrogenic and osteogenic differentiation potential of nestin + -BMSCs. (G) The expression of primary cilia in nestin + -BMSCs was detected by ace-tubulin fluorescence staining. Bar indicated 20 μm. (H) Expression of actin in nestin + -BMSCs stained with TRITC labeled Phalloidin Rhodamine. Bar indicated 50 μm. (I) The expression of acive-YAP in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (J) The expression of TAZ in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (K & L) Expression of key components of Hippo signaling pathway in nestin + -BMSCs. *, p < 0.05; #, p < 0.01.

    Journal: Journal of Advanced Research

    Article Title: The effects of primary cilia-mediated mechanical stimulation on nestin + -BMSCs during bone-tendon healing

    doi: 10.1016/j.jare.2024.09.012

    Figure Lengend Snippet: (A to C) The transverse and longitudinal migration ability of nestin + -BMSCs were measured by scratch assay and transwell assay, respectively. (D) The proliferative activity of nestin + -BMSCs was detected by CCK8. (E & F) Alcian blue staining, alizarin red staining and gene expression levels detected by qRT-PCR were used to analyze the chondrogenic and osteogenic differentiation potential of nestin + -BMSCs. (G) The expression of primary cilia in nestin + -BMSCs was detected by ace-tubulin fluorescence staining. Bar indicated 20 μm. (H) Expression of actin in nestin + -BMSCs stained with TRITC labeled Phalloidin Rhodamine. Bar indicated 50 μm. (I) The expression of acive-YAP in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (J) The expression of TAZ in nestin + -BMSCs was detected by immunofluorescence. Bar indicated 50 μm. (K & L) Expression of key components of Hippo signaling pathway in nestin + -BMSCs. *, p < 0.05; #, p < 0.01.

    Article Snippet: The expression of IFT88 and key components of Hippo/YAP signaling pathway (p-LATS: CST, #8654; p-YAP: CST, #13008; active-YAP: CST, #29495; Total YAP: CST, #4912, p-TAZ: CST, #59971, TAZ: CST, #83669; 14–3-3 protein: CST, #8312) in nestin + -BMSCs of each group were detected by WB assay.

    Techniques: Migration, Wound Healing Assay, Transwell Assay, Activity Assay, Staining, Gene Expression, Quantitative RT-PCR, Expressing, Fluorescence, Labeling, Immunofluorescence

    (A & B) Inhibitory effect of actin on active-YAP and TAZ expression in nestin + -BMSCs. Bar indicated 50 μm. (C & D) Inhibitory effect of actin on expression of key components of Hippo signaling pathway in nestin + -BMSCs. (E) The effect of inhibiting Hippo signaling pathway on nestin + -BMSCs’ proliferation activity was detected by CCK8. (F to H) The effects of Hippo signaling inhibition on nestin + -BMSCs’ migration were detected by scratch assay and transwell assay. (I & J) The effects of Hippo signaling inhibition on nestin + -BMSCs’ differentiation potential were detected by alcian blue staining, alizarin red staining and gene expression levels. *, p < 0.05; #, p < 0.01.

    Journal: Journal of Advanced Research

    Article Title: The effects of primary cilia-mediated mechanical stimulation on nestin + -BMSCs during bone-tendon healing

    doi: 10.1016/j.jare.2024.09.012

    Figure Lengend Snippet: (A & B) Inhibitory effect of actin on active-YAP and TAZ expression in nestin + -BMSCs. Bar indicated 50 μm. (C & D) Inhibitory effect of actin on expression of key components of Hippo signaling pathway in nestin + -BMSCs. (E) The effect of inhibiting Hippo signaling pathway on nestin + -BMSCs’ proliferation activity was detected by CCK8. (F to H) The effects of Hippo signaling inhibition on nestin + -BMSCs’ migration were detected by scratch assay and transwell assay. (I & J) The effects of Hippo signaling inhibition on nestin + -BMSCs’ differentiation potential were detected by alcian blue staining, alizarin red staining and gene expression levels. *, p < 0.05; #, p < 0.01.

    Article Snippet: The expression of IFT88 and key components of Hippo/YAP signaling pathway (p-LATS: CST, #8654; p-YAP: CST, #13008; active-YAP: CST, #29495; Total YAP: CST, #4912, p-TAZ: CST, #59971, TAZ: CST, #83669; 14–3-3 protein: CST, #8312) in nestin + -BMSCs of each group were detected by WB assay.

    Techniques: Expressing, Activity Assay, Inhibition, Migration, Wound Healing Assay, Transwell Assay, Staining, Gene Expression